Create a polyline region of interest:

1. Select the "Polyline" tool in the list of annotations and ROIs (See "How to create 2D annotations")





2. Bring your mouse above one image in a display area and click the left button to define the first point of the polyline.
3. Add a new point for your polyline by dragging the mouse to a new location and click the left button.
4. Double click on the left button of the mouse to add the last point of the polyline.

Modify the polyline position:

1. Select the polyline object by clicking on it with the left mouse button. When a polyline object is selected, some green circles are displayed around its points.
2. Keep your mouse above the polyline (outside any green circles), press the left mouse button and hold it.
3. While holding the left mouse button, drag your mouse to move the location of the polyline.
4. Release the left button mouse.

Modify the position of a point:

1. Select the polyline object by clicking on it with the left mouse button. When a polyline object is selected, some green circles are displayed around its points.
2. Keep your mouse above one of the green circle, press the left button of the mouse and hold it.
3. While holding the left button, drag your mouse to modify the position of the selected point of the polyline.
4. Release the left button of the mouse.

Modify the properties of the polyline:

1. Select the polyline object by clicking on it with the left mouse button. When a polyline object is selected, you can see a green circle at each extremity of its segments.
2. In the properties window of annotations and ROIs, modify the color or the width of the line object.